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. Author manuscript; available in PMC: 2010 Aug 23.
Published in final edited form as: J Neurochem. 2002 Mar;80(6):980–988. doi: 10.1046/j.0022-3042.2002.00778.x

Fig. 3.

Fig. 3

Alpha tubulin binds directly to mGluR7a. Protein overlay blots probed with anti-GST antibody show that the GST-mGluR7a CT fusion protein binds specifically to purified tubulin (Tub, 10 μg; 60 kDa) and calmodulin (CaM, 1 μg; 17 kDa), but not to bovine serum albumin (BSA, 10 μg; 66 kDa) (a). Control experiments show that the GST fusion protein (1 μg; 27 kDa) does not bind to tubulin or calmodulin, but is specifically recognized by the anti-GST antibody (b).