Table 3.
ELISPOT Responses to HIV-1 Epitopes Derived from the Nef Protein
| Peptide ID | Peptide sequence | Peptide sequence D2 isolate | Spotsa |
|---|---|---|---|
| NEG | 0 | ||
| NEG | 0 | ||
| nef-5139 | MGGKWSKRSVVGWPT | MGGKWSKSSIVGWPA | 0 |
| nef-5140 | WSKRSVVGWPTVRER | WSKSSIVGWPAVRDR | 0 |
| nef-5141 | SVVGWPTVRERMRRA | SIVGWPAVRDRMRRA | 270 |
| nef-5142 | WPTVRERMRRAEPAA | WPAVRDRMRRAEPAA | 350 |
| nef-5156 | PVRPQVPLRPMTYKA | PVRPQVPLRPMTFKA | 410 |
| nef-5157 | QVPLRPMTYKAAVDL | QVPLRPMTFKAAVDL | 10 |
| nef-5158 | RPMTYKAAVDLSHFL | RPMTFKAAVDLSHFL | 110 |
| nef-5160 | VDLSHFLKEKGGLEG | VDLSHFLKEMGGLDG | 0 |
| nef-5161 | HFLKEKGGLEGLIYS | HFLKEMGGLDGLIYS | 0 |
| nef-5162 | EKGGLEGLIYSQKRQ | EMGGLDGLIYSQRRQ | 0 |
| nef-5169 | FPDWQNYTPGPGIRY | FPDWQNYTPGPGTRF | 0 |
| nef-5170 | QNYTPGPGIRYPLTF | QNYTPGPGTRFPLTF | 0 |
| nef-5171 | PGPGIRYPLTFGWCF | PGPGTRFPLTFGWCF | 450 |
| nef-5177 | VEEANEGENNSLLHP | VEEANEGETNNLLSP | 0 |
| nef-5178 | NEGENNSLLHPMSLH | NEGETNNLLSPMSLH | 0 |
| nef-5179 | NNSLLHPMSLHGMDD | TNNLLSPMSLHGMED | 0 |
| nef-5180 | LHPMSLHGMDDPERE | LSPMSLHGMEDPEKE | 0 |
| nef-5181 | SLHGMDDPEREVLEW | SLHGMEDPEKEVLVW | 0 |
| nef-5184 | LEWKFDSRLAFHHMA | LEWKFDSRLAFHHIA | 0 |
| nef-5185 | FDSRLAFHHMARELH | FDSRLAFHHIAKELH | 0 |
| nef-5186 | LAFHHMARELHPEYY | LAFHHIAKELHPEYY | 0 |
a
Spot forming units/million PBMCs.
The CTL responses of the childís PBMCs against selected overlapping peptides from Clade B nef consensus sequence peptides1 were determined by ELISPOT assay. Sequences of the synthetic peptides used in the assay (peptide sequence) and the sequences derived from the child's second isolate are presented. Amino acid residues in the child's viral peptide seqences distinct from the consensus sequences are in bold.