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. Author manuscript; available in PMC: 2010 Aug 23.
Published in final edited form as: Muscle Nerve. 2009 Aug;40(2):279–286. doi: 10.1002/mus.21371

Figure 2.

Figure 2

Immunohistochemical studies of AChR-α expression and ligand-binding ability at the mouse muscle endplate. Cryosections of mouse limb muscle were double-stained with tetramethylrhodamine-conjugated α-BTx (first column) and goat polyclonal antibody against AChR-α1 (second column); merge on the right. Representative data from each experimental group (Control, EAMG mild, and EAMG severe) are shown. Mice with severe EAMG show a high level of aberrant AChR protein expression (i.e., expression separate from functional ACh binding as determined by absence of α-BTx binding). Mice with mild EAMG either have normal (expression only in association with α-BTx binding) or very low levels of aberrant AChR protein expression at the NMJ. The immunofluorescence data shown represent one of at least 15 sites analyzed for each experimental group with approximately 2–3 endplates visualized per site. Magnification for all images is 63×10×2.5; bars are 20 μm. All images were captured under similar conditions.