Figure 6.
FRET screening of 1,4-disubstituted 1,2,3-triazole binding affinity for model RNAs AM1A and C11U. The relative fluorescence intensity change ΔF was calculated by ΔF=[(F−F0)/F0]*100, where F is the fluorescence intensity with ligand and F0 is without ligand at 585 nm upon excitation at 467 nm. All of the compounds were tested at a final concentration of 10 µM.