Fig. 2.
Cre expression is specific to chondrocyte cells. Image presented is the PCR product of Cre and GAPDH for primary chondrocytes (C) and osteoblasts (O). We amplified 30 and 15 ng of RNA for 40 cycles for Cre and GAPDH, respectively. Cre expression was not detectable in primary osteoblast cells by PCR analysis or real-time RT-PCR.