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. 2010 Aug 23;5(8):e12333. doi: 10.1371/journal.pone.0012333

Figure 5. Long-term NAC treatment affects sub-cellular localization of NFκB.

Figure 5

A. 2.5 µg of cytoplasmic protein lysate from the cortex of 3 alanine- and 3 NAC-treated PDGFb-SNCA mice was run on a 10-well 4–15% SDS-PAGE gel (Bio-Rad). After transfer, the membrane was cut around the 50 kD marker and probed with anti- NFκB p65 antibody (Santa Cruz) and anti-β-actin antibody (Santa Cruz). 2.5 µg of nuclear protein lysate was run on a separate 10-well 4–15% SDS-PAGE gel and the treated in the manner outlined above. In both cases a single 65 kDa band was observed for NFκB and a single 43 kDa band was observed for β-actin. B. Band quantification of cytoplasmic NFκB normalized to β-actin levels. C. Band quantification of nuclear NFκB normalized to Histone H3 levels. Data were analyzed using a 2-tailed Student's t-test. All relevant statistically significant comparisons are indicated on the graphs. The experiment was repeated three times; representative results from a 1 minute exposure of both blots are shown.