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. 2010 Aug 9;16:1514–1524.

Table 2. PCR primer sequences for TYR, P, TYRP1, MATP, and GPR143.

PCR primers PCR primer sequence (5’-3’) Melting temperature (°C) PCR product size (bp)
Tyrosinase (Tyr) gene [18,19]
Exon 1-1
F-CAAACTGAAATTCAATAACATATAAG
63
678
 
R-GTGGACAGCATTCCTTCTCC
 
 
Exon 1-2
F-TTCAGAGGATGAAAGCTTAAGATAAA
62
521
 
R-CGTCTCTCTGTGCAGTTTGG
 
 
Exon 1-3
F-CTGGCCATTTCCCTAGAGC
59
605
 
R-CCACCGCAACAAGAAGAGTC
 
 
Exon 1-4
F-CATCTTCGATTTGAGTGCCC
60
514
 
R-CCCTGCCTGAAGAAGTGATT
 
 
Exon 1-5
F-CACCCATGTTTAACGACATCA
59
225
 
R-GCCAGTCCCAATATGGAATA
 
 
Exon 1-6
F-GACTCTTCTTGTTGCGGTGG
60
252
 
R-CCCTGCCTGAAGAAGTGATT
 
 
Exon 2
F-CCAACATTTCTGCCTTCTCC
60
442
 
R-TCAGCTAGGGTCATTGTCGAT
 
 
Exon 3
F-AGTTATAAATCAAATGGGATAATC A
60
296
 
R-ACATTTGATAGGCACCCTCT
 
 
Exon 4
F-CTGTTTCCAATTTAGTTTTATAC
55
790
  
R-TACAAAATGGCCTATGTTAAGC
 
 
Exon 5
F-TGTCTACTCCAAAGGACTGT
59
924
 
R-GGCACTTAGCTGGATGTGTT
 
 
Pink eyed dilution (P) gene [20]
Exon 1
F-GAGTTCTTACTTCGA
52
172
 
R-TAAACCCTCCCTGCCTGTTC
 
 
Exon 2
F-GGTGCAAACGTTAGTCTCAG
60
359
 
R-CCAATCTGTGTGAAGTCCAC
 
 
Exon 3
F-CTGGGAACACATACATTATT
56
209
 
R-GTGCAATGCTCAGAAACTCT
 
 
Exon 4
F-AAGCTTGCTTTGTAGCCATT
65
300
 
R-CGATGTGCGGCCACCGCTGC
 
 
Exon 5
F-GAAAAGTGTCTGAGTCTGGC
62
177
 
R-GTCCCGAAGGTGCCTGGGTT
 
 
Exon 6
F-ATTTATACCTTACTGCTCTC
58
183
 
R-TTTCAGATCTCAGCCAGGCG
 
 
Exon 7
F-GGACATGGGGTTTCTCCTGT
59
264
 
R-TGAGATGAAATGAGATTTCAC
 
 
Exon 8
F-AGATCCCAGATGGTGTCTCA
59
213
 
R-AGGTCAGACTCCTTTAAACG
 
 
Exon 9
F-AGAGGGAGGTCCCCTAACTG
63
272
 
R-ATCTCAAGCCTCCCTGACTG
 
 
Exon 10
F-CTTTCGTGTGTGCTAACTCC
57
195
 
R-ACATCTTTGAGCTGACATCC
 
 
Exon 11
F-GCAGCGCTTCATTAGGCTCA
64
201
 
R-GGCCAGAGAAGGCCCGGTTA
 
 
Exon 12
F-GTCGTTTTAATATGGTGGCC
56
256
 
R-CCTGCAGAAGCAACCTTTA
 
 
Exon 13
F-GCCTCTGTTCTACGAGCCTG
63
231
 
R-TGCCAGAACCTGGCCGCAA
 
 
Exon 14
F-TTCACGATGTGTATAGTGGG
59
239
 
R-AAGTGGAGGTGTGCGTTTAC
 
 
Exon 15
F-GATTACAGGCGTGAGCCACC
61
293
 
R-ACCCATCAACAGATACTTCC
 
 
Exon 16
F-GAGGGTGTTGCTGATATCTG
60
260
 
R-GAATGTTCTGCTGCACACCA
 
 
Exon 17
F-AGGCTCCAAGTCACAGACCG
62
219
 
R-CTTCTTGGAGAAGTGAATCAG
 
 
Exon 18
F-AGTTGCGTAGGTTATGACAC
58
235
 
R-CCCATCCAGAATGTGACAAA
 
 
Exon 19
F-GTTATGTATTTGCAGCCCCT
58
197
 
R-AATCCACCAAATACAATTGA
 
 
Exon 20
F-GAATCGGTGTGTTAACAGTG
59
266
 
R-GTAGGCTTTCTTCATTCACC
 
 
Exon 21
F-GCCTACCTTATGTTCACGTC
58
212
 
R-AATCAAAGAACAGTGGCTGG
 
 
Exon 22
F-TGGTGGGTCTGACCCTAAGT
59
219
 
R-AGGCTATGTCCAGGCTAAAG
 
 
Exon 23
F-ACAGTATGGCAGCTTCTCTG
59
229
 
R-ACTAACTGTTGCTTTGGGCT
 
 
Exon 24
F-GAGAACAGAAGCTTACCACC
55
204
 
R-GCTTAGGAACTAGACAGTTTA
 
 
Exon 25
F-CGTATCTCATGAGCTTATCC
58
574
 
R-AGCATACAATTTGAATGCTG
 
 
Tyrosinase-related protein 1 (TYRP1) gene [21]
Exon 1
F-AGAAGTTCATCAGAGACATC
50
189
 
R-TCACCATCATTAATTACATT
 
 
Exon 2
F-CGTGCTTCAGTCTTCTCTACA
59
490
 
R-GCAAGGACTTATGAACTCATTC
 
 
Exon 3
F-CGCAAGGCAGATGTTTTCATG
59
416
 
R-AAGGCATCTTGTCTGTAAAGA
 
 
Exon 4
F-AGACCAAACAGAAATGAATA
47
305
 
R-AAATTCTGACTCCAAGCTATC
 
 
Exon 5
F-AAAGAGCGACAATAAGAACTC
50
319
 
R-AAAGCCTTCTCAAAGAAACTT
 
 
Exon 6
F-TTGCTATTACCTGGAAAAGTG
51
275
 
R-TGCAAAAAGCATATGAAAATG
 
 
Exon 7
F-ATACGTTGTCTTTGGAATAAT
51
252
 
R-ATACCGTGATTACTCTACTTG
 
 
Exon 8
F-TGTCCACTTTTTGGTGATAAC
50
323
 
R-ATTCAACCAGGTGGTTTTGTG
 
 
Membrane-associated transporter protein (MATP) gene [22]
Exon 1-1
F-AGGCTCCACGTCAAATCCAG
63
260
 
R-GGTCACATACGCTGCCTCCA
 
 
Exon 1-2
F-CAGACTCATCATGCACAGCA
58
252
 
R-ATGCCCACGAGCATCATGAC
 
 
Exon 1-3
F-CAGCATTGTGTGGTTCCTCA
58
261
 
R-GGTCAAACACATGAACATCCTC
 
 
Exon 2
F-AACGCGGATGATTCTAAAACAGGA
65
280
 
R-CTCATTGTCTGGGGAGCTGA
 
 
Exon 3-1
F-GGGAGTGTCTATGCATGAGG
65
324
 
R-GATAGAACCATACTCGTACATTCC
 
 
Exon 3-2
F-GCCCCACTTACAGAGGTTGC
63
224
 
R-CAACAAAGAGCAAGAATATTTTCCCTTG
 
 
Exon 4
F-AGCTGGCTGAGTTTCTGCAG
62
265
 
R-CCTCAACAGGTGTTAATGGAGG
 
 
Exon 5
F-AGAGGTGGAGAAGCAGAGTG
64
236
 
R-GAAGACATCCTTAGGAGAGAG
 
 
Exon 6
F-ATGAGGCACTGCCAGCTGTA
64
286
 
R-CCCAAGGCAGAGGTTCAATG
 
 
Exon 7
F-GCCCTAAATGACAGTTCCTTG
58
326
 
R-TGTGCTTCACTGTCTCTGAG
 
 
G protein-coupled receptor 143 (GPR143) gene [23]
Exon 1-1
F-GAGCCTGGCTCTACTGCAGGCGCT
64
250
 
R-TGCCCAGGCAGAGCGCGTGGAAGG
 
 
Exon 1-2
F-AGCCACGCAGCTCGTGCTGAGCTTCCAGCC
68
250
 
R-CCCAGGCGCTGATCAGATTCCAACCCGCG
 
 
Exon 2
F-TCATTTTTCCAAAGCAAGAAGTCAGC
66
293
 
R-GCAGGACGTGAGAACCTGCATT
 
 
Exon 3
F-GTCTACCCTGCCGTCTCAAGGATG
66
248
 
R-CGCTCAGTGCCATCTCTTATCTTCC
 
 
Exon 4
F-GTTCCAGGCAGGCCTCTGTGC
68
229
 
R-GGCTCATGTATTCCCTGCAAGACAAC
 
 
Exon 5
F-TTTCCCTTTTTGTTCTCATCCTCTTA
63
299
 
R-AGGACAACATGTGTCACTGTCTGAG
 
 
Exon 6
F-ACCTGCTTCCATTGCCTTCTCTGTC
68
288
 
R-CTTCCCTTTGGAACTTCTGGTCACG
 
 
Exon 7
F-GAAATTCTTCTCTGACTCTCCAGCATT
63
278
 
R-TGACAGAGTGAGACCTTGTCTCTGA
 
 
Exon 8
F-ATGGTCCCTTCCAAGCGAGTCC
68
492
 
R-TCACATGAGAGGTGCTGCTGAACAC
 
 
Exon 9
F-TGAAAAACTCCATGCACTGAATACT
61
597
  R-TGCATAACTGTACATGTATTTATTTTCTTTTG