Table 2. PCR primer sequences for TYR, P, TYRP1, MATP, and GPR143.

PCR primers	PCR primer sequence (5’-3’)	Melting temperature (°C)	PCR product size (bp)
Tyrosinase (Tyr) gene [18,19]
Exon 1-1	F-CAAACTGAAATTCAATAACATATAAG	63	678
	R-GTGGACAGCATTCCTTCTCC
Exon 1-2	F-TTCAGAGGATGAAAGCTTAAGATAAA	62	521
	R-CGTCTCTCTGTGCAGTTTGG
Exon 1-3	F-CTGGCCATTTCCCTAGAGC	59	605
	R-CCACCGCAACAAGAAGAGTC
Exon 1-4	F-CATCTTCGATTTGAGTGCCC	60	514
	R-CCCTGCCTGAAGAAGTGATT
Exon 1-5	F-CACCCATGTTTAACGACATCA	59	225
	R-GCCAGTCCCAATATGGAATA
Exon 1-6	F-GACTCTTCTTGTTGCGGTGG	60	252
	R-CCCTGCCTGAAGAAGTGATT
Exon 2	F-CCAACATTTCTGCCTTCTCC	60	442
	R-TCAGCTAGGGTCATTGTCGAT
Exon 3	F-AGTTATAAATCAAATGGGATAATC A	60	296
	R-ACATTTGATAGGCACCCTCT
Exon 4	F-CTGTTTCCAATTTAGTTTTATAC	55	790
	R-TACAAAATGGCCTATGTTAAGC
Exon 5	F-TGTCTACTCCAAAGGACTGT	59	924
	R-GGCACTTAGCTGGATGTGTT
Pink eyed dilution (P) gene [20]
Exon 1	F-GAGTTCTTACTTCGA	52	172
	R-TAAACCCTCCCTGCCTGTTC
Exon 2	F-GGTGCAAACGTTAGTCTCAG	60	359
	R-CCAATCTGTGTGAAGTCCAC
Exon 3	F-CTGGGAACACATACATTATT	56	209
	R-GTGCAATGCTCAGAAACTCT
Exon 4	F-AAGCTTGCTTTGTAGCCATT	65	300
	R-CGATGTGCGGCCACCGCTGC
Exon 5	F-GAAAAGTGTCTGAGTCTGGC	62	177
	R-GTCCCGAAGGTGCCTGGGTT
Exon 6	F-ATTTATACCTTACTGCTCTC	58	183
	R-TTTCAGATCTCAGCCAGGCG
Exon 7	F-GGACATGGGGTTTCTCCTGT	59	264
	R-TGAGATGAAATGAGATTTCAC
Exon 8	F-AGATCCCAGATGGTGTCTCA	59	213
	R-AGGTCAGACTCCTTTAAACG
Exon 9	F-AGAGGGAGGTCCCCTAACTG	63	272
	R-ATCTCAAGCCTCCCTGACTG
Exon 10	F-CTTTCGTGTGTGCTAACTCC	57	195
	R-ACATCTTTGAGCTGACATCC
Exon 11	F-GCAGCGCTTCATTAGGCTCA	64	201
	R-GGCCAGAGAAGGCCCGGTTA
Exon 12	F-GTCGTTTTAATATGGTGGCC	56	256
	R-CCTGCAGAAGCAACCTTTA
Exon 13	F-GCCTCTGTTCTACGAGCCTG	63	231
	R-TGCCAGAACCTGGCCGCAA
Exon 14	F-TTCACGATGTGTATAGTGGG	59	239
	R-AAGTGGAGGTGTGCGTTTAC
Exon 15	F-GATTACAGGCGTGAGCCACC	61	293
	R-ACCCATCAACAGATACTTCC
Exon 16	F-GAGGGTGTTGCTGATATCTG	60	260
	R-GAATGTTCTGCTGCACACCA
Exon 17	F-AGGCTCCAAGTCACAGACCG	62	219
	R-CTTCTTGGAGAAGTGAATCAG
Exon 18	F-AGTTGCGTAGGTTATGACAC	58	235
	R-CCCATCCAGAATGTGACAAA
Exon 19	F-GTTATGTATTTGCAGCCCCT	58	197
	R-AATCCACCAAATACAATTGA
Exon 20	F-GAATCGGTGTGTTAACAGTG	59	266
	R-GTAGGCTTTCTTCATTCACC
Exon 21	F-GCCTACCTTATGTTCACGTC	58	212
	R-AATCAAAGAACAGTGGCTGG
Exon 22	F-TGGTGGGTCTGACCCTAAGT	59	219
	R-AGGCTATGTCCAGGCTAAAG
Exon 23	F-ACAGTATGGCAGCTTCTCTG	59	229
	R-ACTAACTGTTGCTTTGGGCT
Exon 24	F-GAGAACAGAAGCTTACCACC	55	204
	R-GCTTAGGAACTAGACAGTTTA
Exon 25	F-CGTATCTCATGAGCTTATCC	58	574
	R-AGCATACAATTTGAATGCTG
Tyrosinase-related protein 1 (TYRP1) gene [21]
Exon 1	F-AGAAGTTCATCAGAGACATC	50	189
	R-TCACCATCATTAATTACATT
Exon 2	F-CGTGCTTCAGTCTTCTCTACA	59	490
	R-GCAAGGACTTATGAACTCATTC
Exon 3	F-CGCAAGGCAGATGTTTTCATG	59	416
	R-AAGGCATCTTGTCTGTAAAGA
Exon 4	F-AGACCAAACAGAAATGAATA	47	305
	R-AAATTCTGACTCCAAGCTATC
Exon 5	F-AAAGAGCGACAATAAGAACTC	50	319
	R-AAAGCCTTCTCAAAGAAACTT
Exon 6	F-TTGCTATTACCTGGAAAAGTG	51	275
	R-TGCAAAAAGCATATGAAAATG
Exon 7	F-ATACGTTGTCTTTGGAATAAT	51	252
	R-ATACCGTGATTACTCTACTTG
Exon 8	F-TGTCCACTTTTTGGTGATAAC	50	323
	R-ATTCAACCAGGTGGTTTTGTG
Membrane-associated transporter protein (MATP) gene [22]
Exon 1-1	F-AGGCTCCACGTCAAATCCAG	63	260
	R-GGTCACATACGCTGCCTCCA
Exon 1-2	F-CAGACTCATCATGCACAGCA	58	252
	R-ATGCCCACGAGCATCATGAC
Exon 1-3	F-CAGCATTGTGTGGTTCCTCA	58	261
	R-GGTCAAACACATGAACATCCTC
Exon 2	F-AACGCGGATGATTCTAAAACAGGA	65	280
	R-CTCATTGTCTGGGGAGCTGA
Exon 3-1	F-GGGAGTGTCTATGCATGAGG	65	324
	R-GATAGAACCATACTCGTACATTCC
Exon 3-2	F-GCCCCACTTACAGAGGTTGC	63	224
	R-CAACAAAGAGCAAGAATATTTTCCCTTG
Exon 4	F-AGCTGGCTGAGTTTCTGCAG	62	265
	R-CCTCAACAGGTGTTAATGGAGG
Exon 5	F-AGAGGTGGAGAAGCAGAGTG	64	236
	R-GAAGACATCCTTAGGAGAGAG
Exon 6	F-ATGAGGCACTGCCAGCTGTA	64	286
	R-CCCAAGGCAGAGGTTCAATG
Exon 7	F-GCCCTAAATGACAGTTCCTTG	58	326
	R-TGTGCTTCACTGTCTCTGAG
G protein-coupled receptor 143 (GPR143) gene [23]
Exon 1-1	F-GAGCCTGGCTCTACTGCAGGCGCT	64	250
	R-TGCCCAGGCAGAGCGCGTGGAAGG
Exon 1-2	F-AGCCACGCAGCTCGTGCTGAGCTTCCAGCC	68	250
	R-CCCAGGCGCTGATCAGATTCCAACCCGCG
Exon 2	F-TCATTTTTCCAAAGCAAGAAGTCAGC	66	293
	R-GCAGGACGTGAGAACCTGCATT
Exon 3	F-GTCTACCCTGCCGTCTCAAGGATG	66	248
	R-CGCTCAGTGCCATCTCTTATCTTCC
Exon 4	F-GTTCCAGGCAGGCCTCTGTGC	68	229
	R-GGCTCATGTATTCCCTGCAAGACAAC
Exon 5	F-TTTCCCTTTTTGTTCTCATCCTCTTA	63	299
	R-AGGACAACATGTGTCACTGTCTGAG
Exon 6	F-ACCTGCTTCCATTGCCTTCTCTGTC	68	288
	R-CTTCCCTTTGGAACTTCTGGTCACG
Exon 7	F-GAAATTCTTCTCTGACTCTCCAGCATT	63	278
	R-TGACAGAGTGAGACCTTGTCTCTGA
Exon 8	F-ATGGTCCCTTCCAAGCGAGTCC	68	492
	R-TCACATGAGAGGTGCTGCTGAACAC
Exon 9	F-TGAAAAACTCCATGCACTGAATACT	61	597
	R-TGCATAACTGTACATGTATTTATTTTCTTTTG