Table 3. Canine ADAM9 primer sequences, locations, and product sizes observed by retinal RT–PCR from crd3-affected and normal dogs.

Primer pair	Forward primer name	Forward primer sequence	Forward primer location	Reverse primer name	Reverse primer sequence	Reverse primer location	Observed size in normal (bp)	Observed size in affected (bp)
1	ADAM9_cDNA_F3	GTTGAGTGGAACCTGCGGAATCT	5′ UTR	ADAM9_cDNA_R3	GGCTCTTCTTCTTCATAGTTTGTGG	Exon 6	610	610
2	ADAM9_cDNA_F2	ATTCATCCATTGCTCTCAGCGACT	Exon 5	ADAM9_cDNA_R2	TACAAAGTTCCCCAACACATCACC	Exon 9	482	482
3	ADAM9_cDNA_F4	TGTCCTGCCACAGACCCGATA	Exon 7	ADAM9_cDNA_R4	TGGAACATCACATTCGTTGGTTTT	Exon 14	832	832
4	ADAM9_cDNA_F1	GAGTGTGAATCGGACCCTTGTTGT	Exon 13	ADAM9_cDNA_R1	CATTTGGTGCCTTTACTGGGAGTC	Exon 16	497	NO
5	ADAM9_cDNA_F6	CTGTATTTGGAATCGTGCCTGCTA	Exon 16	ADAM9_cDNA_R6	TACTTTCGGTTGTGGTGGAGGTG	Exon 22	647	NO
6	ADAM9_cDNA_F5	TCAGATGGCAAAAATCAAGCAAAA	Exon 20	ADAM9_cDNA_R5	ATCCATGTTCGGTGCATTAACTTC	3′ UTR	541	541
7	ADAM9_cDNA_F7	TGTCAGCCAGATGTTTTTATTCAGA	Exon 14	ADAM9_cDNA_R7	GTCCACACTTCCTCCGTATCCTTT	Exon 18	573	283
8	ADAM9_cDNA_F8	TTGTGGTTTCTCTGGCAATGA	Exon 15	ADAM9_cDNA_R8	CACTTCCTCCGTATCCTTTAGTCTCA	Exon 18	389	NO
9				ADAM9_cDNA_exon1R	GATATAAGACACCTGTTCTGAATAGG	Exon 1

NO=not observed.