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. 2010 Aug 23;5(8):e12348. doi: 10.1371/journal.pone.0012348

Figure 1. Homozygous knockout mutant line mrs2-10.

Figure 1

(a) A homozygous T-DNA insertion mutant for AtMRS2-10 (At1g80900) was identified by PCR based on the SALK_100361.41.30.x line. (b) RNA extracted from mrs2-10 and Col-0 leaves was tested for the presence of MRS2-10 mRNA with gene-specific primers. Alpha-(α)-Tubulin (TUB) was used as a constitutive control.