Figure 3. Conversion activity in the CAA following Benzonase treatment of UBH or IBH.
A) The CAA was performed in the presence of the indicated concentrations of MgCl2 and B) performed following pre-treatment as indicated. Conversion activity was determined as the fold increase in immunoreactive signal of treated samples relative to their equivalent KO reactions after overnight incubation at 37°C and treatment with PK (100µg/ml, 1hr at 37°C). Quantification is based on at least three experiments, mean and SEM are shown. **p<0.001 or *p<0.05 using one-way analysis of variance (ANOVA) with Dunnet's test for multiple comparisons against the indicated control (GraphPad, Prism).