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. 2010 Aug 23;5(8):e12351. doi: 10.1371/journal.pone.0012351

Figure 5. Binding of wild-type and mutant PrPC expressed in RK-13 to sGAG.

Figure 5

A) The ability of wildtype (101P) and mutant (101L) moPrP expressed in RK-13 cells to bind heparin sepharose beads in the presence of increasing concentrations of NaCl (50, 100, 125, 300, 1000mM), was determined by western blot analysis. N shows neat input. B) PrPC bound to heparin sepharose in the presence of 50mM NaCl was treated with PNGaseF before western blot analysis. Full length (F) and truncated (T) PrP species are shown. C) The percentage of 101P (dotted line) and 101L (solid line) moPrP bound to heparin sepharose in increasing concentrations of NaCl was determined relative to binding in 50mM NaCl. Blots developed with 03R19. Molecular weight (kDa) is shown. Western blots are representative of replicated experiments, quantification is based on at least three experiments, mean and SEM are shown. Binding differed significantly by Two-way ANOVA (p<0.001).