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. 2010 Aug 23;5(8):e12343. doi: 10.1371/journal.pone.0012343

Figure 4. shRNA-mediated AMPK knockdown inhibits the lymphocyte-facilitation of tight junction assembly.

Figure 4

(a) Representative pictures of ZO-1 relocation to cell–cell junctions in (A) Vector control cells; (B) Co-cultured vector control cells; (C) shRNA AMPK cells and (D) Co-cultured shRNA AMPK cells at various time points (0h, 1h, 2h, 3h) after calcium switch. (Scale bar: 30 µm). (b) Quantification of ZO-1 relocation to cell–cell junctions (length of ZO-1/cell (µm)) in culture conditions as indicated in (a) at various time points (0h, 1h, 2h, 3h) after calcium switch. The asterisks denote significant differences detected in (B) co-cultured vector control cells vs. (D) co-cultured shRNA AMPK cells by Student's t test (*, p<0.05; **, p<0.01).