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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Regul Pept. 2010 Jun 10;164(2-3):113–119. doi: 10.1016/j.regpep.2010.06.001

Figure 4. Effects of TM4 peptides on calcitonin receptor BRET.

Figure 4

Shown are the static BRET (A) and saturation BRET ratios (B) for COS cells expressing tagged rCTR constructs in the presence or absence of TM4 peptides from the calcitonin receptor and the secretin receptor. The static BRET signal was significantly reduced with the calcitonin receptor TM4 peptide to a level not different from background. This indicates disruption of rCTR oligomers. Saturation BRET curves (plotted as ratios of YFP fluorescence to Rlu luminescence) in the absence or presence of TM4 peptide (10−5M) were obtained with a fixed amount of donor and increasing amounts of acceptor. The curve generated in the presence of the calcitonin receptor TM4 peptide was not significantly different from a linear fit, consistent with disruption of the complex. The data are presented as means ± S.E.M. of data from four independent experiments performed in triplicate.