Figure 3.
Reversible photochemical activation of a potassium ion (K+) channel. (a) Schematic of PAL-gated K+ channel. The sphere labeled with a plus sign represents a quaternary ammonium group, which blocks the channel when the diazobenzene is in the trans conformation. Irradiation at 380 nm switches the diazobenzene from trans to cis, thus enabling K+ flow. Irradiation with 500 nm light reverses the switching event, thus blocking the ion channel. (b) Light-controlled membrane potential of a PAL-gated K+ channel measured in dependence of light irradiation.