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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Protein Expr Purif. 2010 May 11;74(1):16–23. doi: 10.1016/j.pep.2010.04.021

Figure 1.

Figure 1

Structure and operon organization of A) cholera toxin and B) plasmid pCTD002 for LcrV-CTA2/B chimera expression. The CTA2 domain that is incorporated with fusion proteins in CT-like chimeras corresponds to amino acids 195–240 (blue). The toxic CTA1 domain not present in CT chimeras corresponds to amino acids 1–194 (light grey). CTB pentamer is shown with three subunits (dark grey). The ER-targeting KDEL motif at the C-terminus is shown in red. The lcrV incorporated into pCTD002 is from Y. enterocolitica and is truncated at nucleotide 812 and thus amino acid 270 to remove the C-terminal 54 amino acids of LcrV (lcrV*).