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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Protein Expr Purif. 2010 May 11;74(1):16–23. doi: 10.1016/j.pep.2010.04.021

Figure 3.

Figure 3

SDS-PAGE and western blot analysis of the purification of the LcrV-CTA2/B chimeras. A) SDS-PAGE of d-galactose agarose elutions 1 and 2 of the Y.enterocolitica LcrV-CTA2/B chimera (pCTD002). B) Western blot analysis of the Y.enterocolitica chimera using α-CTA, α-CTB and α-LcrV antibodies. C) SDS-PAGE of d-galactose agarose elutions 1 and 2 of the Y. pestis LcrV-CTA2/B chimera (pCTD007) from 25 ml of starting culture. Bands at 38kD represent the LcrV-CTA2 fusion, and bands at 11.5kD represent CTB monomeric subunits.