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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Protein Expr Purif. 2010 May 11;74(1):16–23. doi: 10.1016/j.pep.2010.04.021

Figure 5.

Figure 5

Confocal microscopy of LcrV-CTA2/B trafficking on tissue culture cells. Cells were incubated with LcrV-CTA2/B from Y. pestis for 45 minutes at either 4°C to inhibit endocytosis or 37°C to promote uptake and compared to CT trafficking. A) CT with α-CT primary antibody on Vero cells, B) LcrV-A2/B with α-CT primary antibody on Vero cells, C) LcrV-CTA2/B with α-LcrV primary antibody on Vero cells and D) LcrV-A2/B with α-LcrV primary antibody on J774 cells.