Fig. 3. Hydrogen/deuterium exchange in Q70E and Q162E compared to WT βB2-crystallin.
The relative deuterium incorporation (Da) (Eq. 1, Methods) at 15 sec, 1 min, 4 min, 15 min, 60 min and 24 h are shown for peptic peptides from WT, Q70E, and Q162E. The 8 representative peptides contain residues A) 1–21, B) 53–70, C) 71–81, D) 94–110, E) 111–126, F) 117–131, G) 143–164, and H) 151–164.