Fifty million TRITC-labeled lymphocytes from 3-4-wk-old female NOD mice were transferred i.v. into age-matched female NOD mice. Two h after cell transfer, donor B cells in PanLN, PLN, MLN and PP of host mice were identified by immunofluorescence staining with a FITC-anti-B220 mAb followed by FACS analysis. Representative FACS plots show donor B cells (TRITC+B220+ cells in box) in LNs and PP of host mice. Numbers in FACS plots are mean ± standard derivation of donor B cells as a percentage of total lymphocytes. One-way ANOVA test, *p<0.05 and **p<0.01 compared with PanLN, n=4 mice in each group.