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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Exp Eye Res. 2010 Jun 22;91(3):393–404. doi: 10.1016/j.exer.2010.06.013

Fig. 4.

Fig. 4

Identification of homozygous AQP1 transgenic AQP0 knockout (TgAQP1+/+/AQP0-/-) mice by genotyping. PCR-based genotyping of heterozygous AQP0 knockout (AQP0+/-), homozygous AQP0 knockout (AQP0-/-), heterozygous AQP1 transgenic AQP0 knockout (TgAQP1+/-/AQP0+/-), and homozygous AQP1 transgenic AQP0 knockout (TgAQP1+/+/AQP0-/-) mice. Samples were loaded as follows: lanes 1 WT; lane 2, AQP0+/-; lane 3, AQP0-/-; lane 4, TgAQP1+/-/AQP0+/-; lane 5, TgAQP1+/+/AQP0-/-; lane 6, molecular weight marker. White open arrow, 277 bp segment; black arrow, 788 bp segment; arrowhead, 1.9 kb segment.