Fig. 5.

LRRK2 overexpression leads to dopamine deficiency. Dopamine levels in transgenic worms expressing GFP reporter alone or together with LRRK2 WT, R1441C and G2019S were measured on adult day 4. Non-transgenic N2 worms and cat-2(e1112) mutant worms deficient in dopamine synthesis were used as controls. The dopamine contents were normalized with total proteins present in each strain (left axis) and percent dopamine was shown relative to N2 (right axis). Synchronized worms grown to adult day 4 were collected for dopamine measurements using reverse phase HPLC with electrochemical detection. Error bars indicate SEM. *p<0.05 and **p<0.01 compared to N2.