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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Neurobiol Dis. 2010 May 26;40(1):193–199. doi: 10.1016/j.nbd.2010.05.024

Figure 6.

Figure 6

Rapamycin reduces mossy fiber sprouting. Rats with spontaneous recurrent seizures were treated with rapamycin or vehicle for three weeks. Photomicrographs of Timm staining were acquired from brain slides prepared from naïve rats (A), rats with spontaneous recurrent seizures (B) and rats with spontaneous seizure treated with rapamycin (C). The dense band of mossy fibers in the supragranular layer (marked by * in panel D) and measured by drawing a contour along the midline of the granular cell layer (g) and the inner third of the inner molecular layer (im). The optical intensity in the square box in panel D was used to measure background staining. (E) Data are presented as average intensity of Timm staining in the contour area (control: 5.4 ± 1.07; pilocarpine: 27.03 ± 3.51; Pilocarpine plus rapamycin: 9.01± 1.42) from 5 sets of animals. *p<0.05 by t-test (mean ± SE).