Figure 1.

Electron cryomicrograph of rotavirus SA11–4F TLPs embedded in vitreous ice under various chemical conditions. (a) TNC (10 mM Tris, 150 mM NaCl, 10 mM CaCl₂, pH 7.5). (b) 250 mM NH₄OH (pH 11.5); arrow indicates condensed RNA in the center of each particle. (c) 3-[cyclohexylamino]-1-propanesulfonic acid) (CAPS) buffer, pH 11.6. (d) 250 mM NH₄Cl in TNC (pH 7.5). (e) TNC (pH 9.0) after NH₄OH treatment (pH 11.5). (f) TNC (pH 7.5) after NH₄OH treatment (pH 11.5). (Scale bar: 1,000 Å.)