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Transcription assay of normal (pH 7.5) and ammonium high pH-treated DLPs. (a) High-resolution polyacrylamide gel (PAGE) analysis of transcription reaction mixtures containing native DLPs (Untreated) and DLPs brought back to native conditions after ammonium high pH treatment (Treated). The middle lane contained no particles. (b) RNA yield in transcription assay at 0, 15, and 30 min. (c) Quantitation of RNA yield after 30 min.
