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. 2010 Apr 12;38(15):5130–5140. doi: 10.1093/nar/gkq198

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Published by Oxford University Press (2010).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Effect of downstream exons on processing to sFlt1. A series of minigene constructs containing exons 13, 14 and 15 with intervening sequence in various combinations were transfected into JEG-3 cells and Flt1 splicing, and sFlt1 processing were analyzed by qRT–PCR. (A and C) Products of PCR reactions analyzed by gel electrophoresis. Schematic representation above each lane demonstrates sequences within minigene construct and schematic to the right indicates the position of primers used. When exon 13 and intron 13 sequences are present, splicing downstream is inhibited with multiple products of low abundance seen (upper portion of A and C). Transfected minigenes are however efficiently processed to sFlt1 (lower portion of A and C) when exon 13 and intron 13 are present. (B and D) Effect of exon 15 on sFlt1 processing compared to exon 14. Exon 15 significantly increases processing to sFlt1.