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. 2010 Apr 12;38(15):4929–4945. doi: 10.1093/nar/gkq200

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Methylation acquisition during spermatogenesis and absence of methylation imprints in Dnmt3a-difecient spermatogonia. (A) Methylation status of three Gpr1-Zdbf2 DMRs in E14.5, E16.5 and E18.5 prospermatogonia. As a control, the paternally methylated H19 DMR and the maternally methylated Lit1 DMR were included. The regions we analyzed and the CpG sites in this study are shown in Figure 2A. (B) Methylation profile of DMRs in normal and Dnmt3a-deficient prospermatogonia. Male germ cells at P7 were isolated from testes of normal and the conditional Dnmt3a knockout mice by FACS. The bisulfite-PCR-based assays for the three paternally methylated Gpr1-Zdbf2 and the H19 DMRs and maternally methylated Lit1 DMR.