Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Apr 12;38(15):4929–4945. doi: 10.1093/nar/gkq200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Paternal allele-specific methylation at the region between GPR1 and ZDBF2 of human chromosome 2 and imprinting of human GPR1. (A) Structure of the human region between GPR1 and ZDBF2. Two methylated regions, DMRh1 and DMRh2, identified in meDIP-on-chip of normal human sperm indicated by filled boxes. The vertical bars represent CpG sites. The horizontal arrows represent primer positions. The extent of the regions analyzed in this study and Genbank accession numbers are shown over the line. (B) Bisulfite-PCR sequencing of genomic DNA prepared from cord blood, placenta and two cases’ sperm. Each row represents a unique methylation profile within the pool of 20 clones sequenced. Closed and open circles represent methylated and unmethylated CpGs, respectively. (C) The paternal-specific expression of GPR1 in human samples. The A/G polymorphic site (SNP ID: rs3755227) in GPR1 exon 3 is indicated by vertical arrow. Heterozygosity was demonstrated in DNA isolated from cord blood with double peaks in chromatographic sequencing data at the polymorphic residues identified (arrow).