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MMS gradient plate assays to assess the effects of deleting DDT pathway genes on HK578-10D pol30Δ::HIS3 cells harboring pol30·UB gene fusions. (A) In the pol30Δ rad18Δ strain background; (B) in the pol30Δ ubc13Δ background; (C) in the pol30Δ rev3Δ background; and (D) in the pol30Δ ubc13Δ rev3Δ background. Experimental conditions were as described in Figure 2.
