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. 2010 Apr 12;38(15):4958–4969. doi: 10.1093/nar/gkq244

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — PRC2 activity regulates H3K27Ac levels. (A) Western blot analyses of histones purified from WT, Eed^−/−, Suz12^−/−, Ezh2 conditional (Ezh2 loxP/loxP) and Ezh2^−/− ES cells using the indicated antibodies. H3 is presented as loading control. (B) ChIP analysis of the Olig2 promoter in WT, Suz12 and Eed KO ES cells using the indicated antibodies. Signals are normalized to histone density using an H3-specific antibody. (C) Western blot analyses using the indicated antibodies and luciferase activity of 293T cells containing a stable integration of a heterologous Gal4/luciferase reporter construct before and after Gal4-EZH2 expression. (D) ChIP analysis of the luciferase TSS in the cells presented in (C) using the indicated antibodies. Gal4, EZH2 and SUZ12 enrichments are presented as percentage of input while the different histone modifications signals are normalized to histone density using an H3-specific antibody.