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Regulation of H3K27me3 and H3K27Ac target gene binding in MLL-AF9 HSPCs and FDCP-mix cells. (A) β-Globin, Lipocalin and Hoxa9 qPCR expression analyses in FDCP-mix cells before and after granulocytic differentiation and in MLL-AF9-expressing HSPC. (B) ChIP analyses of Hoxa9 and Olig1 promoters in FDCP-mix cells and MLL-AF9-expressing HSPCs using the indicated antibodies. H3K27Ac, H3K9Ac and H3K27me3 signals are normalized to histone density using an H3-specific antibody.
