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. 2010 Apr 12;38(15):4958–4969. doi: 10.1093/nar/gkq244

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — p00 and Cbp are required for efficient H3K27 acetylation in Suz12 KO ES cells. (A) qPCR expression analyses of the indicated genes in Suz12 KO ES cells transfected for 48 h with the indicated siRNA oligos. ‘U’ indicates the control siRNA oligo carrying a scrambled oligoribonucleotide sequence. (B) Western blot analyses of histones purified from Suz12 KO ES cells transfected with the indicated siRNA oligos using the indicated antibodies. H3 is presented as loading control. Quantification of the H3/H3K27Ac signal is indicated above each lane. A scrambled siRNA oligo (SCR) was used as negative control. (C and D) Western blot analyses of protein extracts and of purified histones from Suz12 KO ES cells transfected with the indicated siRNA oligos using the indicated antibodies. Vinculin, Ponceau staining and H3 are presented as loading controls. A scrambled siRNA oligo (SCR) was used as negative control. Quantification of the H3/H3K27Ac signal of western blots presented in ‘C’ is indicated above each lane. (E) Average quantification of the H3/H3K27Ac signals between the two independent siRNA experiments presented in C and D.