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. 2010 Apr 12;38(15):4958–4969. doi: 10.1093/nar/gkq244

Figure 6.

Figure 6.

Inhibition of p300 and Cbp activities leads to downregulation of H3K27 acetylation. (A) Western blot analyses of histones purified from WT and two independent Suz12 KO ES cell lines cultured in the presence (+) or absence (−, DMSO) of AA using the indicated antibodies. Ponceau staining of histones is presented as loading control. (B) Western blot analyses of histones purified from Suz12 KO ES cell lines cultured in the presence (+) or absence (−, DMSO) of AA using the indicated antibodies. H3 is presented as loading control. (C) Schematic representation of a model for the competition between PcG and p300/Cbp activities during ES cell differentiation and HSPC immortalization. TF, transcription factor.