Figure 5.

Evidence for two AFs in c-Myb. CV-1 cells were transfected with a Myb-responsive reporter plasmid containing three or four MREs (0.2 µg) as indicated and (A) 0.4 µg of plasmids encoding either full-length c-Myb, its SUMO-conjugation negative mutant c-Myb-2KR, or a deletion variant c-Myb ΔNRD, or (B) full-length c-Myb, a variant with the central transactivation domain deleted (c-Myb-ΔT1) and SUMO-negative c-Myb with TAD deleted (c-Myb-ΔT1-2KR). The results are presented as RLU ± SEM. Western controls shown in (A) and (B) were analysed using anti-c-Myb (5E11) and anti-GAPDH antibodies. The numbers under each lane/bar correspond to the constructs shown in the lower boxes, V: empty vector. (C) CV-1 cells were transfected with reporters containing one or four Myb responsive elements [1×MRE(GG)-MYC or 4×MRE(GG)-MYC] and plasmids encoding c-Myb-ΔT1 or c-Myb-ΔT1-2KR. The results are presented as SF ± SEM.