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. 2010 Apr 12;38(15):4970–4984. doi: 10.1093/nar/gkq245

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — The ID in Sp3 harbours a SRAF. CV-1 cells were transfected with 0.2 µg of plasmids expressing Gal4p-DBD fused to (A) Sp3 (amino acid residues 81–613) wild-type or the kee SUMO-negative mutant, or (C) Sp3 ID (amino acid residues 534–612) wild-type and kee mutant. The reporter output from the E1b-driven Gal4p-responsive reporter plasmid (5×GRE, 0.2 µg) was normalized to the effect of Gal4p-DBD (0.2 µg) and set to 100. The results are presented as RLU ± SEM. (B and D) Based on parallel transfections, using an 1×GRE-E1b-Luc reporter plasmid (0.2 µg), the SFs for the GBD-Sp3 and GBD-Sp3-ID constructs assayed in (A) and (C) were calculated. The results are presented as SF ± SEM.