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The importance of the number of AFs for level of synergy. CV-1 cells were transfected with reporter plasmids containing one or four MREs (1×MRE(GG)-MYC or 4×MRE(GG)-MYC) and an effector plasmid encoding one of the following c-Myb variants: c-Myb, c-Myb-ΔNRD encoding amino acids 1–443, the SUMO-conjugation negative mutant c-Myb-2KR, as well as c-Myb and c-Myb-2KR fused C-terminally to the herpes simplex virus VP16 transactivation domain. The results are presented as SF ± SEM.
