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. 2010 Apr 19;38(15):5242–5259. doi: 10.1093/nar/gkq258

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 10. — Single strands of native or modified agRNAs are inactive. (A) Sequences: passenger strands are given 5′-3′, guide strands are given 3′-5′ as shown. For these agRNAs the passenger strand is the antisense strand. Modification codes: dna, RNA, 2′F-RNA, 2′Ome-rna, lna; p = phosphate. (B) PR-9 duplex and native or modified single-strands. (C) PR-11 duplex and native or modified single-strands. All data are normalized to the guide strand of mismatch control MM4. RNA levels are the average (±SD) from at least two independent transfections. Protein levels were also confirmed by at least two independent transfections, from which a typical western blot is shown.