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. 2010 Apr 19;38(15):5242–5259. doi: 10.1093/nar/gkq258

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 14. — Inactive modified duplexes do not compete for target sites. Western analysis showing no competition between native agRNAs and inactive modified duplexes applied to cells in two transfections 4 days apart. (A) Native PR-9 silences gene expression equally effectively whether it is transfected before or after an inactive chemically modified duplex (or negative control). (B) Native PR-11 activates gene expression equally effectively whether it is transfected before or after an inactive chemically modified duplex.