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. 2010 Apr 19;38(15):5242–5259. doi: 10.1093/nar/gkq258

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — PR gene expression can be activated by agRNAs containing 2′F-RNA. (A) Duplex sequences: passenger strands are listed on top in all cases; for agRNAs PR-11 and PR-22 this is the antisense strand. Modification codes: dna, RNA, 2′F-RNA; p = phosphate. (B) PR-11 analogs containing one strand heavily or fully modified with 2′F-RNA. (C) PR-11 analogs containing a minimally 2′F-RNA-modified guide strand. (D) PR-22 analogs containing one or both strands heavily or fully modified with 2′F-RNA. All data are normalized to mismatch control MM4. RNA levels are the average (±SD) from at least two independent transfections. *P < 0.05, **P < 0.01, ***P < 0.005. Protein levels were also confirmed by at least two independent transfections, from which a typical western blot is shown.