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. 2010 Apr 19;38(15):5242–5259. doi: 10.1093/nar/gkq258

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — PR gene expression can be silenced by agRNAs containing combinations of modifications. (A) Duplex sequences: passenger strands are listed on top in all cases; for agRNA PR-9 this is the antisense strand. LNA sequences contain 5-methylcytosine instead of cytosine. Modification codes: dna, RNA, 2′F-RNA, 2′Ome-rna, lna; p = phosphate. (B) PR-9 analogs containing combinations of previously identified active strands. All data are normalized to mismatch control MM4. RNA levels are the average (±SD) from at least two independent transfections. Protein levels were also confirmed by at least two independent transfections, from which a typical western blot is shown.