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. 2010 Apr 19;38(15):5059–5074. doi: 10.1093/nar/gkq271

Figure 4.

Figure 4.

Defective repair of DSBs induced by γ-rays and camptothecin in GEMIN2-deficient cells. (A) Time line of γ-H2AX foci per cell at the indicated time after treatment with 4Gy γ-rays. Results for WT cells and for GEMIN2−/−tetGEMIN2 untreated (GEMIN2+) and treated (GEMIN2−) with doxycycline for 4 days are shown. Error bars represent standard deviation. Statistical analysis was performed using the t test. (B) Ionizing-radiation-induced chromosome aberrations. Mitotic cells were harvested at 6 h after exposure of an asynchronous population of cells to 4Gy γ-rays. Irradiated cells were treated with colcemid for the last 3 h before harvest to enrich the mitotic cells. Results from WT and GEMIN2−/−tetGEMIN2 cells untreated with doxycycline (GEMIN2+) and treated (GEMIN2−) with doxycycline for 4 days are shown. The number of chromosomal aberrations per 50 mitotic cells is shown on the Y-axis. Error bars represent standard deviation. (C) Time course of the formation of γ-H2AX foci per cell at the indicated times after treatment with camptothecin (CPT, 100 ng). Results for WT cells and for GEMIN2−/−tetGEMIN2 untreated (GEMIN2+) and treated (2 days, GEMIN2− 2D; 3 days, GEMIN2− 3D; 4 days, GEMIN2− 4D) with doxycycline are shown. CPT is supposed to induce DSBs only in cells at the S phase, and the number of γ-H2AX foci was counted only in γ-H2AX foci positive cells. Error bars represent standard deviation.