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. 2010 Jul 29;2010:295394. doi: 10.1155/2010/295394

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Copyright © 2010 Wanderley de Souza et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunofluorescence microscopy colocalization (arrows) of phosphorylated proteins (b, g), detected using antiphosphotyrosine antibody, actin filaments (c), detected using phalloidin, and PI3 kinase (f) detected using anti-PI3 Kinase antibody during penetration of T. cruzi into macrophages. The overlay images (d, h) show the areas of colocalization demonstrating that phosphorilated proteins and microfilaments participate in internalization of T. cruzi trypomastigotes by macrophages. Bars = 5 μm (after [9]).