Figure 4.

Imunofluorescence microscopy localization of GM1 (a–d) and flotillin 1 (e-f) during internalization of T. cruzi trypomastigotes by macrophages suggests the participation of membrane microdomins in this process. (a–d) colocalization of GM1, using cholera toxin subunit B (a) and an intracellular parasite ((c): arrow). (b) shows labeling of the nucleus and kinetoplast labeled with propidium iodide. (c) corresponds to a DIC image; (d) is a merge image. (e-f): Colocalization of flotillin1 (a), detected using a specific antibody, and trypomastigotes ((b): arrows) bars – 5 μm (after [16]).