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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jul 30;878(25):2348–2352. doi: 10.1016/j.jchromb.2010.07.019

Table 1.

LC method for the focus mode HTLC online extraction method

Loading Pump Eluting Pump
Step Start Sec Flow (mL/min) Grad A% B% C% D% Tee Loop Flow (mL/min) Grad A% B%
1 0.00 60 2.00 Step 100.0 - - - ==== out 0.70 Step 100.0 -
2 1.00 120 0.10 Step 100.0 - - - T in 0.60 Step 100.0 -
3 3.00 90 2.00 Step - 100.0 - - ==== in 0.70 Ramp 5.0 95.0
4 4.50 90 2.00 Step - 75.0 25.0 - ==== in 0.70 Step 5.0 95.0
5 6.00 160 2.00 Step 100.0 - - - ==== out 0.70 Step 100.0 -