Fig. 4.

Kctd15 inhibits NC induction by antagonizing the Wnt signal. (A,B) Kctd15 suppressed NC-specific marker gene induction by Chrd + Wnt3a, but not by Chrd + β-cat* in Xenopus animal caps. Injected RNAs are indicated at the top; expression of slug, foxd3, sox9 and of odc as control was assayed by RT-PCR. (C) Wnt signaling in HEK293T cells was measured by TOPflash luciferase. Kctd15 moderately inhibited Wnt signaling stimulated by Wnt3a or LiCl but not by β-cat*. AC, uninjected animal caps; −RT, without reverse transcriptase; RLU, relative luciferase units.