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. 2010 Sep 15;137(18):3107–3117. doi: 10.1242/dev.046573

Fig. 2.

Fig. 2.

An HLH54F-lacZ reporter recapitulates the expression of HLH54F. Wild-type Drosophila embryos hybridized with HLH54F digoxygenin RNA (A,C,E,G) and comparable HLH54Fb-lacZ embryos stained with β-galactosidase (β-gal) antibodies (B,D,F,H). (A,B) Blastoderm stage embryo (stage 5, A) and embryo at early gastrulation (stage 6, B). (C,D) Stage 9 embryos during germ band elongation. (E,F) In stage 13 embryos, the cells expressing HLH54F mRNA (E) and β-gal (F) have migrated anteriorly and spread along either side of trunk visceral mesoderm. (G) Stage 15 embryo with fluorescent detection of HLH54F mRNA, showing newly formed and elongating syncytia distributed over the midgut. (H) Stage 16 embryo showing HLH54F-lacZ-stained longitudinal muscle fibers.