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. 2010 Sep 15;137(18):3107–3117. doi: 10.1242/dev.046573

Fig. 6.

Fig. 6.

HLH54F and Bin as co-regulators of a caudal visceral mesoderm-specific enhancer from the Doc locus. (A-H) lacZ (A-D) or nuclear GFP (E-H) reporter gene expression from DocF4s1 and derivatives is shown in early stage 12 Drosophila embryos as detected with anti-β-gal (diaminobenzidine) and anti-GFP (fluorescence) antibodies (dorsal views). (A) DocF4s1-lacZ in a wild-type background showing expression in bilateral CVM clusters at the beginning of cell migration (right). Additional activity is seen in bilateral clusters of the procephalic neuroectoderm (left). (B-D) DocF4s1-lacZ in embryos with UAS-HLH54F (B), UAS-bin (C) and UAS-HLH54F + UAS-bin (D), driven pan-mesodermally by twi-GAL4. (E) Control DocF4s1-GFP expression. (F) DocF4s1 Hmut-GFP expression (all E-boxes mutated). (G) DocF4s1 Bmut-GFP expression (all Bin binding motifs mutated). (H) DocF4s1 H+Bmut-GFP (all HLH54F and Bin binding motifs mutated). (I) Map of the DocF4s1 enhancer within the Doc locus and of HLH54F (H) and Bin (B) binding motifs within the enhancer.