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. 2010 Sep 15;137(18):3107–3117. doi: 10.1242/dev.046573

Fig. 7.

Fig. 7.

Regulation of early HLH54F expression. HLH54F mRNA staining of late blastoderm stage Drosophila embryos (A,C,E,G,I,K,M,P) and in stage 11 embryos (B,D,F,H,J,L,N,Q,S,R) of wild type and mutants as indicated. (A,B) Wild-type HLH54F expression. (C,D) Slightly reduced HLH54F expression and mis-migrating CVM cells at stage 11 in twi mutants. (E,F) Complete absence of HLH54F expression in sna mutants. (G,H) Posterior expansion of HLH54F expression at blastoderm stage and increased CVM at stage 11 in hkb mutants. (I,J) Almost complete absence of HLH54F expression at blastoderm and absence of HLH54F-expressing CVM at stage 11 in tll mutants. (K-N) Reduced HLH54F expression domains in byn and fkh mutant blastoderm embryos and reduced HLH54F-labeled CVM at stage 11. (O) Schematic of the expression domains of the tested genes at late blastoderm stage. (P) Early gastrulation stage fkh byn double mutant showing only low, residual levels of HLH54F expression (arrowhead). (Q) Stage 11 fkh byn double mutant with very few HLH54F-expressing cells (arrowhead). (R) Early stage 12 wild-type embryo co-stained for HLH54F mRNA (green) and Zfh1 protein (red) in the migrating CVM. (S) Stage 12 zfh1-deficient embryo stained as in R. HLH54F is expressed in CVM cells, but cell migration is disrupted.