Figure 1.

Generation of ATF2^KI mice. (A) Scheme outlines strategy used to replace serines 472/480 with alanines on a genomic clone used for homologous recombination. Location of the loxP-PGK-neo-loxP cassette is indicated. DTA is a negative selection marker. (B) Restriction fragment-based genotyping of ATF2^KI mice. Noted is one of the two MwoI sites lost upon S472A substitution. Representative ethidium bromide–stained gel depicts the different PCR garments obtained following MwoI digestion for each of the possible genotypes.