Figure 2.

ATF2KI mice are more sensitive to ionizing radiation (IR). (A) Kaplan-Meier survival analysis. Age-comparable adult wild-type (ATF2^WT) and knock-in (KI) animals received 8Gy whole-body irradiation. Thirty days after irradiation, 75% of the ATF2^KI group died, while 70% of the animals in the ATF2^WT group survived the irradiation using Kaplan-Meier survival analysis. (B) Terminal transferase-mediated dUTP nick end-labeling (TUNEL) analysis of spleen from ATF2^WT and ATF2^KI animals obtained 3 or 24 hours after 8Gy IR or corresponding controls. (C) Fluorescence-activated cell sorting (FACS) analysis of thymus and spleen from ATF2^WT and ATF2^KI animals performed 48 hours after exposure to 5Gy IR. Shown are representative data (n = 4 for ATF2^WT, n = 5 for ATF2^KI).