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. 2010 May;1(5):421–433. doi: 10.1177/1947601910375274

Figure 3.

Figure 3.

IL-13.E13K-D2-NLS travels to and very efficiently accumulates in the nuclei of GBM cells, IL-13Rα2 overexpressors. (A) Conjugation of IL-13.E13K-D2-NLS and IL-13.E13K-D2 to Alexa Fluor 488. The primary amines in the IL-13.E13K-D2-NLS and the IL-13.E13K-D2 were conjugated directly to the tetrafluorophenyl (TFP)-reactive Alexa Fluor 488 dye. The labeled proteins were run on 12% SDS-PAGE gel and stained with Coomassie blue. (B) As in A, but the blot scanned using Typhoon 9210 to detect fluorescent proteins. (C and D) Alexa Fluor 488 labeled IL-13.E13K-D2-NLS localizes to the nucleus of U-251 MG GBM cells. The intracellular localization of IL-13.E13K-D2-NLS labeled with Alexa Fluor 488 in U-251 MG cells was examined using confocal microscope. (E) As in C and D, but the protein used was control IL-13.E13K-D2. The data are representative of 3 independent experiments.